Volume 5, Issue 2, June 2020, Page: 22-28
Oral Toxicity Assessment and In vitro Antimicrobial Profile of Methanolic Leaf Extract of Alchornea Cordifolia on Albino Rats
Komolafe Kafilat Adenike, Department of Science Laboratory Technology, Microbiology Unit, the Oke-Ogun Polytechnic, Saki, Shaki, Nigeria; Department of Zoology, Parasitology Unit, University of Ibadan, Ibadan, Nigeria
Adeoti Olatunde Micheal, Department of Science Laboratory Technology, Microbiology Unit, the Oke-Ogun Polytechnic, Saki, Shaki, Nigeria; Department of Pure and Applied Biology, Ladoke Akintola University of Technology, Ogbomosho, Nigeria; Department of Zoology, Cellular Parasitology Unit, University of Ibadan, Ibadan, Nigeria
Olaoye Opeyemi Joy, Department of Science Laboratory Technology, Microbiology Unit, the Oke-Ogun Polytechnic, Saki, Shaki, Nigeria
Olufemi Olutope Samson, Department of Science Laboratory Technology, Microbiology Unit, the Oke-Ogun Polytechnic, Saki, Shaki, Nigeria
Adedokun Elizabeth Olajumoke, Department of Science Laboratory Technology, Microbiology Unit, the Oke-Ogun Polytechnic, Saki, Shaki, Nigeria
Adedoja Sulaiman Aderogba, Department of Science Laboratory Technology, Microbiology Unit, the Oke-Ogun Polytechnic, Saki, Shaki, Nigeria
Adesina David Ademola, Department of Science Laboratory Technology, Microbiology Unit, the Oke-Ogun Polytechnic, Saki, Shaki, Nigeria
Abiola Adebiyi Oladepo, Department of Science Laboratory Technology, Microbiology Unit, the Oke-Ogun Polytechnic, Saki, Shaki, Nigeria
Received: Dec. 22, 2019;       Accepted: Jan. 6, 2020;       Published: Aug. 5, 2020
DOI: 10.11648/j.eeb.20200502.12      View  133      Downloads  24
Abstract
The ameliorative tendency of the leaves of Alchornea cordifolia has been reported against ailments ranging from conjunctivitis to yaws and certain parasitic infections. This necessitated investigating the in vitro antibacterial efficacy of methanol-extracted leaves of Alchornea cordifolia on hematological and histopathological of organ of toxicity on albino rats. The rats were randomly segregated into four groups of five animals in each cage. The groups were orally administered with 250, 500 and 750 mg/kg body weight and 10% Tween 80 control for 28 days. Blood samples were collected for hematological analysis and organs (liver and spleen) for histopathological analysis. The data obtained were analyzed by ANOVA and Dunnett’s test at P>0.05 levels of significance. Methanol leaf extract had a significantly higher inhibitory zone in E. coli and K. pneumonia ranging 35.00± 1.73 and 35.67± 3.48 at all the concentrations tested. There was no significant effect on hematological parameters. Liver necrosis was noticed in the harvested organs of the experimental rats. The liver sections of rat treated with 750mg/kg of the leaf extract showed cloudy swelling of hepatocytes and mild Kupffer cell hyperplasia. These results suggest that Alchornea cordifolia is non-toxic but has the propensity to induce hepatic injury at high doses. Conclusively, successful antibacterial activity at all concentrations and the slight pathological effects could be indicative low toxicity and high efficacy of this plant if taken at lower doses.
Keywords
Alchornea Cordifolia, Hyperplasia, Conjunctivitis, Antibacterial, Histopathological
To cite this article
Komolafe Kafilat Adenike, Adeoti Olatunde Micheal, Olaoye Opeyemi Joy, Olufemi Olutope Samson, Adedokun Elizabeth Olajumoke, Adedoja Sulaiman Aderogba, Adesina David Ademola, Abiola Adebiyi Oladepo, Oral Toxicity Assessment and In vitro Antimicrobial Profile of Methanolic Leaf Extract of Alchornea Cordifolia on Albino Rats, Ecology and Evolutionary Biology. Vol. 5, No. 2, 2020, pp. 22-28. doi: 10.11648/j.eeb.20200502.12
Copyright
Copyright © 2020 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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